Assay of chloramphenicol in clinical specimens.
نویسندگان
چکیده
منابع مشابه
Comparison of methods available for assay of chloramphenicol in clinical specimens.
Eight methods for the assay of chloramphenicol in clinical samples were compared with our own modification of a plate diffusion technique using Sarcina lutea and yeast extract agar. Six of the eight methods were less sensitive than originally reported, and five of them were considered unsuitable for use in clinical microbiology practice. The remaining three methods together with the S. lutea/ye...
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A simplified radioenzymatic assay for chloramphenicol was developed by eliminating the need for cumbersome extraction procedures. After the acetylation of chloramphenicol with [(14)C]acetyl coenzyme A in the presence of chloramphenicol acetyltransferase, the reaction mixture was added to a toluene-based scintillation fluid. Since (14)C-acetylated chloramphenicol is more soluble than [(14)C]acet...
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Group testing, where groups of individual specimens are composited to test for a binary outcome, is a procedure used to reduce the costs of screening large numbers of individuals. Applications of group testing include Bovine Viral Diarrhea virus detection in cattle herds (Kennedy et al., 2006), blood donation screening (Dodd et al., 2002), discovery of chemical compounds to use in new drugs (Re...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1981
ISSN: 0021-9746
DOI: 10.1136/jcp.34.2.225-a